transforming growth factor beta-induced is essential for endotoxin tolerance induced by a low dose of lipopolysaccharide in human peripheral blood mononuclear cells
نویسندگان
چکیده
our prior study found that transforming growth factor beta-induced (tgfbi) is an important negative regulator in tlr-induced inflammation. however, whether tgfbi may affect inflammation during lipopolysaccharide (lps)-induced endotoxin tolerance (et) is still unclear.this study aimed to investigate whether tgfbi was involved in the mechanisms of et in human through dampening nuclear factor-kappa b (nf-κb) mediated pathway. et models of isolated healthy volunteers peripheral blood mononuclear cells (pbmcs) were established by pretreating with a low dose of lps to observe the changes of tgfbi expression during et induction, compared with ten healthy controls. moreover, a vector-based short hairpin rna expression system was used to specifically inhibit tgfbi expression to further explore its role in et induction. the expression was analyzed by reverse transcription-polymerase chain reaction (rt-pcr) and western blotting. the responses to lps were determined by the activation of nf-κb, the production of tumor necrosis factor-α (tnf-α) and nitric oxide (no), which were analysed by enzyme-linked immuno sorbent assay (elisa).the results showed that tgfbi expression in the et group obviously increased; et also led to a hyporesponse of pbmcs to lps with less activation of nf-κb, less production of tnf-α and no, as well as more expression of tgfbi than those of non-et group. moreover, the inhibitory effect was partly refracted in plasmid tgfbi short hairpin rna (ptgfbi-shrna) transfected pbmcs. meanwhile, the absence of tgfbi caused abnormal enhancement of inflammatory cytokine production and it was involved in et induction through dampening nf-κb mediated pathway.therefore, tgfbi may be a new target for the clinical treatment of inflammatory disorders.
منابع مشابه
Transforming Growth Factor Beta-Induced Is Essential for Endotoxin Tolerance Induced by a Low Dose of Lipopolysaccharide in Human Peripheral Blood Mononuclear Cells.
Our prior study found that transforming growth factor beta-induced (TGFBI) is an important negative regulator in TLR-induced inflammation. However, whether TGFBI may affect inflammation during lipopolysaccharide (LPS)-induced endotoxin tolerance (ET) is still unclear. This study aimed to investigate whether TGFBI was involved in the mechanisms of ET in human through dampening nuclear factor-kap...
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عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۴، شماره ۳، صفحات ۳۲۱-۳۳۰
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